Metabolism of 2,5-dimethyl-4-hydroxy-3(2H)-furanone in detached ripening strawberry fruits

1997 
The transformation of S-[methyl-14C]adenosyl-l-methionine (14C-SAM) and [2-(or 5-)methyl-14C]-2,5-dimethyl-4-hydroxy-3(2H)-furanone (14C-1) was investigated in detached ripening strawberry fruits over a three-day period. Radiochemical analysis of the different fruit parts revealed that 46% and 51% of the applied 14C remained in the stems for 14C-SAM and 14C-1, respectively. In samples obtained by solid phase extraction, 1, 2,5-dimethyl-4-methoxy-3(2H)-furanone (2), and glycosidically bound 1 were analyzed by reversed-phase high-performance liquid chromatography. Incorporation of 14C-SAM and 14C-1 into 2 was observed with transformation rates of 0.4% and 0.3% of the applied 14C, respectively. In addition, 0.7% of the applied 14C-1 was recovered as glycosidically bound 1. The data support the conclusions that 14C-SAM is the source of the methyl group in the 4-methoxy compound 2 and that 1 is the precursor of 2 and the glycosides studied. Keywords: 2,5-Dimethyl-4-hydroxy-3(2H)-furanone; 2,5-dimethyl-4-methox...
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    15
    References
    37
    Citations
    NaN
    KQI
    []