DNA nanomechanics allows direct digital detection of complementary DNA and microRNA targets

Over the past decade, several techniques have been developed to improve the detection of small amounts of DNA and RNA molecules, but detection of DNA molecules at concentrations below the femtomolar level requires amplified detection schemes. A unique nanomechanical response of hybridized DNA and RNA molecules that serves as an intrinsic molecular label is now reported; nanomechanical measurements allow direct detection and counting of hybridized molecules.