PCR-RFLP for typing of Chinese DEN-2 genotypes

In this study, the prM-E genes of 5 Chinese DEN-2 isolates from Fujian, Guangxi and Hainan were amplified by RT-PCR.Two restriction enzymes were selected by software DNASTAR to generate the polymorphisms in different restriction fragments length for strain discrimination. The digested fragments were visualized by silver staining. The results showed that strain DEN-2 FJ-10 and strain FJ-11 belong to the same genotype,strain D2-43 and strain D2-44 are another genotype and strain D2-04 is different from the former 4 strains. Those results by PCR-RFLP correlate well to those previously determined by sequencing. The PCR-RFLP technology is a useful tool for rapid typing of viral isolates, especially suitable for laboratory test and clinical diagnosis in endemic areas of dengue fever.