High performance liquid chromatography of principal alkaloids in arisaematis

Objective An improved HPLC method is reported for the determination of arisaematis's principal alkaloids, providing a fast and simultaneous determination for this by using a solid phase column. Methods The analysis was performed with fresh extractions of arisaematis from Changbai mountains. To quantify the amount of alkaloids in a fast and reproducible way by means of UV-vis absorption measurements. Results The amount of the alkaloids present in fresh arisaematis extracts was determined by using the chromatographic methodology Conclusions This UV-vis methodology can be extended to the determination of other compounds and herbs if the restrictions mentioned in the text are respected.