Methods of blood glucose measurement for early-stage severely burned rats

Objective To compare the differences of blood glucose detected by four methods with different instruments and specimen types at early stage in severely burned rats. Methods 24 Sprague-Dawley (SD) rats were randomly divided into two groups: control group 1 (Sham scald group, n=8) and scald injury group 1 (n=16) . Blood samples of scald injury group 1 were collected at 12, and 24 hours after scald (n=8, each time) . Another 20 SD rats were randomly divided into two groups: control group 2 (Sham scald group, n=10) and scald injury group 2 (n=10) . Blood samples of scald injury group 2 were collected at 12 hours after scald. The rats in scald injury group 1 and 2 were placed into scalding water (95.0±0.5) ℃ for 15 seconds to model third-degree burn with 30% total burn surface area (TBSA) . The rats in scald injury group 1 were given intraperitoneal injection with normal saline (40 ml/kg) immediately, while those in scald injury group 2 were given intraperitoneal injection with normal saline (40 ml/kg) 6 hours after scald. The rats in Sham scald group 1 and 2 were placed into warm water 37℃ for 15 seconds, and did not received injection. Portable glucometer/caudal artery (vein) blood, portable glucometer/abdominal aorta blood, spectrophotometer/femoral venous plasma, and spectrophotometer/abdominal aorta plasma were used to detect blood glucose. Results ①Compared with Sham scald group 1, the levels of blood glucose detected by portable glucometer/caudal artery (vein) blood and spectrophotometer/femoral venous plasma in scald injury group1 at 12, 24 hours after scald were significantly increased (P 0.05) . The levels of blood glucose detected by portable glucometer/caudal artery (vein) blood were significantly lower than those detected by spectrophotometer/femoral venous plasma (P 0.05) . Conclusion Four kinds of methods used in this study shows that the levels of blood glucose were significantly increased at early stage in severely burned rats, and the portable glucometer/abdominal aorta blood is a relatively simple and fast method to detect blood glucose. Key words: Burn; Rat; Blood glucose