The estimation of aflatoxin M1 in milk using a two-dimensional thin-layer chromatographic method suitable for survey work

Summary An existing method for the analysis of aflatoxin M 1 in milk has been modified by the introduction of two-dimensional thin-layer chromatography and certain confirmatory tests. The improved method is now sufficiently sensitive to demonstrate the presence in cows' milk of aflatoxin M 1 resulting from the ingestion of feed contaminated with aflatoxin B 1 at the very low maximum level permitted by the current UK Fertilizers and Feeding Stuffs Regulations.