Effects of Buffered Deglycerolization Solutions on the 21-Day Post-Thaw Preservation of Red Blood Cells

Abstract : Fresh-frozen or rejuvenated-frozen red cells were deglycerolized by centrifugal washing. The effects of phosphate buffering of the wash solutions on the pH of the washed cell suspension was examined. Buffering raised the red cell pH, and the positive effects of this higher pH were examined when the cells were re-stored at 4 deg C for 21 days in either AS-17 or AS-3 preservation solutions. The results indicate that the buffering will improve the ability of AS-17, and to a lesser extent AS-3, to preserve fresh, or rejuvenated, frozen-thawed red cells.... Blood storage, Frozen blood, 2,3-DPG, ATP, Post-thaw storage, Deglycerolization.