Evaluation of tributyltin resistance in marine sediment bacteria

Tributyltin (TBT) resistance of aerobic and anaerobic bacteria isolated from TBT-contaminated coastal sediments in Arcachon Bay, France, was determined and compared with taxonomically similar bacteria originating from diverse non-TBT-contaminated environments. The minimum inhibitory concentrations (MICs) showed that TBT was toxic to most of the bacteria studied at concentrations above 2.1 μM (0.7 mg/L) for clostridia, 76 μM (25 mg/L) for pseudomonads, and 3 mM (1000 mg/L) for enterobacteria. The aerobic or facultative anaerobic Gram-negative bacteria (pseudomonads and enterobacteria) presented the highest TBT tolerance. No difference in TBT resistance was observed between strains originating from the polluted site and strains originating from non-TBT-polluted environments (thermal muds, Hospital of Bordeaux, etc.), suggesting that a TBT-polluted environment did not select for more specific resistant bacteria. It should be noted that the bacteria tested were found to be tolerant to TBT concentrations 1000 ...