Column chromatography of 5-fluorouracil on sephadex G-10.

Abstract The influence of pH, ionic strength ( I ) and flow-rate of eluent on the chromatographic parameters of 5-fluorouracil (5-FU) on Sephadex G-10 columns was studied. Using an optimized phosphate buffer (pH 7.0, I 0.12), 5-FU showed reversible adsorption on the gel matrix and can be chromatographed in the submicrogram range without losses.