[Protective effect of hawthorn leaf procyanidins on cardiomyocytes of neonatal rats subjected to simulated ischemia-reperfusion injury].

Objective:To study the direct effect of hawthorn leaf procyanidins on cardiomyocytes subjected to ischemia-reperfusion injury and elucidate their therapeutic mechanism on ischemic heart diseases. Method:Cultured cardiomyocytes of neonatal rats were subjected to anoxia-reoxia injury which simulated the ischemia-reperfusion injury in vivo,and hawthorn leaf procyanidins were applied. The therapeutic effect was valued by LDH leakage and MTT test. For a further mechanism study,contents of MDA and activities of SOD in cardiomyocytes were measured. Result:Hawthorn leaf procyanidins in 24-60 mg?L-1 significantly and dose-dependently inhibited LDH leakage( compared with the model group,all P0.01 ) and cell viability decrease( compared with model group,24-48 mg·L-1 groups all P 0.05;60 mg·L-1 group,P0.01) in cardiomyocytes induced by anoxia-reoxia injury. Furthermore,hawthorn leaf procyanidins in 24-60 mg·L-1 significantly inhibited the increase of MDA content(compared with the model group,all P0.01) and the decreased of SOD activity(compared with the model group,24 mg·L-1 group,P0.05;other groups all P0.01) in cardiomyocytes undergoing anoxia-reoxia injury. Conclusion:Hawthorn leaf procyanidins have a significant therapeutic effect on the simulated ischemia-reperfusion injury of cultured neonatal rat cardiomyocytes,which may relate to their anti-oxidation effects. And the direct protective effect of hawthorn leaf procyanidins on cardiomyocytes subjected to ischemia-reperfusion injury may be one of the key mechanisms of its therapeutic effect on ischemic heart diseases.