High-performance liquid chromatographic determination of nitrite ion by use of hydralazine.

Hydralazine reacts with nitrite ion under an acidic condition at 37°to form tetrazolo-[5, 1-a] phthalazine (Tetra-P) almost quantitatively. Without extraction, Tetra-P was determined sensitively by high-performance liquid chromatography. This method is more specific than the conventional methods for the determination of nitrite ion.