Chemical composition, pretreatments and saccharification of Senna siamea (Lam.) H.S. Irwin & Barneby: an efficient biomass producing tree legume.

Abstract Protocols were developed for efficient release of glucose from the biomass of Senna siamea , one of the highly efficient biomass producing tree legumes. Composition of mature, 1 year and 2 years coppice biomass were analysed. For the hydrolysis of the glucan, two pretreatments, cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF) and alkali (sodium hydroxide) were used; COSLIF (85% phosphoric acid, 45 min incubation at 50 °C) pretreated mature biomass exhibited best result in which 88.90% glucose released after 72 h of incubation with the use of 5 filter paper units (FPU) of cellulase and 10 international units (IU) of β -glucosidase per gram of glucan. Of the biomass of different particle sizes (40–200 mesh) used for saccharification, 40–60 mesh shown the maximum glucose release. COSLIF pretreated mature, 1 year and 2 years coppice biomass showed equivalent glucose release profiles.