연구논문 : 샴푸 제형의 in vitro 항비듬력 평가법

Dandruff is a common scalp disorder that affects almost half of population. Cosmetic companies have developed anti-dandruff shampoo containing antimicrobial materials such as zinc pyrithione (ZPT) and climbazole which inhibit fungal growth especially Malassezia yeast, dandruff-causing organism. It is essential to examine whether anti-dandruff shampoo reduces Malassezia growth, so that improve dandruff. The aim of this investigation was to establish a test method for antifungal activity of shampoo. The basic methods of antimicrobial susceptibility testing are the minimum inhibitory concentration (MIC) and disk diffusion test. We have compared a disk diffusion test that uses nitrocellulose filter paper and artificial skin substrate (Vitro-Skin) as disks to estimate antifungal activity of shampoo or materials after rinse-off. We have identified that ZPT, climbazole and three commercial shampoos containing ZPT were deposited on disks with nitrocellulose filter paper and artificial skin substrate (Vitro-Skin) even after rinse-off, so zone of inhibition were observed.