Quantitative Analysis and Preformulation of Extracts from Alnus Japonica

ABSTRACT − Alnus japonica has been known to exert antioxidative, anti-inflammatory, anti-cancer and immune responseinhibitory effects. The aim of this study was to figure out the characteristics of extracts obtained with different extractionsolvent such as water, 100% ethanol, 70% ethanol or 70% methanol because characteristic components such as oregoninand hirsutanone extracted from Alnus japonica might be essential for the biological activity. For this purpose, oregonin andhirsutanon of four extracts, index ingredient of Alnus japonica, were analyzed with HPLC and physicochemical studies suchas SEM, particle size and zeta potential were conducted. In cell cytotoxicity study, extract of water showed the highest cyto-toxicity among four extracts. In case of oregonin, 70% MeOH and water extracts showed high contents and in case ofhirsutanone, all extracts showed similar contents except 70% EtOH extracts. The extract of 70% MeOH from Alnus japonicafor both oregonin and hirsutanone appeared to have the highest content. Both oregonin and hirsutanone extracted from Alnusjaponica using 70% methanol showed stability in pH 1.2.Key words