Soldium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: Comparison of dansylation, Coomassie Blue R-250 and silver detection methods

Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially with basic proline-rich proteins (PRPs).