Effect of Parenteral Lipid Emulsion SMOF Lipid and Intralipid on Redox Potential of Glutathione and DNA Methylation in Newborn Guinea Pig

Infants born Objectives To compare the oxidative property of SM and IL by measuring redox potential of glutathione, and to correlate the DNA methylation values with the redox values. Methods At three day of life, guinea pigs received PN (dextrose, amino acids, vitamins, electrolytes) compounded with SM (n=15) or IL (n=16) through a jugular vein catheter. After 4 days of infusion, redox potential of glutathione (mV, by the Nernst equation using the GSH and GSSG concentrations measured by capillary electrophoresis) and global DNA methylation (U = nmol of 5MedCyt / μg DNA; commercial kit) were determined in liver. Statistics: t test and Pearson correlation; p Results The mean (±sem) redox potential was higher in SM (SM: -207±1, IL: -213±2 mV, p -1 •x – 2.96U; r 2 = 0.37, p Conclusion In our animal model, compared to IL, SM is a more potent oxidizing agent, which could have important impact on health throughout life.