Development of Indirect Competitive Chemiluminescence ELISA Method for the Detection of Fumonisin B1

To develop an indirect competitive chemiluminescence enzyme linked immunosorbent assay(CL-ELISA) for the detection of fumonisin B1(FB1) in corn samples,conjugate antigens with keyhole limpet hemocyanin(KLH)-FB1 and ovalbumin(OVA)-FB1 were synthesized,polyclonal antibody was prepared by immunizing the New Zealand rabbits with KLH-FB1,and OVA-FB1 was used as coating antigen.After optimizing the working conditions and factors,CL-ELISA was established and used to detect FB1 in spiked corn sample with FB1 standard substance.The results showed that,using the CL-ELISA method to determine FB1,the regression equation was Y=0.602 5-0.370 7X(R 2=0.986 2),the detectable range was from 0.14 ng/mL to 33.3 ng/mL with a minimum detection limit of 0.14 ng/mL,and the recovery reached 83.5%~102%.It can be concluded that the method we developed is sensitive and hopefully can be used to the actual determination of FB1 in corn samples.