A Study in Preparation of $^{113m}In$ colloid as Scanning Agent and it's Organ Distribution in Rats

The newly developed diagnostic method with application of cow system ( 118 days, 1.7 hrs, 390 Kev, Single ) has the remarkable advantages such as increased diagnostic ability by single large dose administration of with no subsequent radiation hazard and shortened examining time. We reformed the research of following scope with the use of developed cow (25 mCi) generator: The sizes of particles produced under various conditions were investigated, and possibility for application to the scannings of various organs such as brain, liver, lung, bone marrow and blood pool etc. were studied. Results: solution eluted from diluted HCl solution (pH 1.5) passed through generator, and there can be produced various sized particles of colloidal indium. And there observed the state of distribution of in each organ which showed many differences according to the particle sizes of colloidal indium. The results are stated as follows: 1. The adjustment of pH is the most important factor in making the desirable particle size of colloidal indium. The colloid for blood pool showed the highest level as 7.1%/gm blood, at pH 1.7, the colloid of pH 3.5 for liver scanning showed the highest level, 88.4%, in the liver, the colloid pH 6 showed the highest level, 3.1%, in the spleen, and the colloid of pH 11.0 showed the highest level, 85.3%/gm, in the lung. 2. The colloid for liver scanning made with NaCl-NaOH system showed the highest liver uptake at pH 7.2, and at either higher or lower pH than 7.2 showed decrease of liver uptake more or less. 3. The activity of eluted through generator indicated over 90% in the initial 4 ml, and particularly 88.1%-86.0% in the initial 2 ml. 4. The incubation time, tempertaure and mechanical irritation related to colloid formation and coating of colloid were not the definite condition of influence.