The expressiom of β1 integrin in colons of Hirschsprung disease

Objective To investigate the expression of β1 integrin (ITGB1) in the tissue samples from patients with Hirschsprung's disease (HD).Methods The stenotic,transitional and normal appearance intestines from forty patients with HD were collected during operation.For comparative gene expression studies,the expression levels of β1 integrin was detected using immunohistochemistry,Real-time PCR (RT-PCR) and western blotting.Results Immunohistochemistry studies shows that β1 integrin immunoprecipitation product was mainly localized to the cytoplasm and membrane of the ganglion cells.The mean optical density(OD) value was 0.86 ± 0.16,0.61 ± 0.15,and 0.35 ± 0.07 (give in descending order) in normal,transitional and stenotic segments of HD,respectively,the difference was statistically significant (P ＜ 0.05).The mRNA expression of β1 integrin in stenotic and transitional segments was significantly lower than that in the normal bowel segment (the relative quantification was 0.77 ± 0.17,1.08 ± 0.15,2.26 ± 0.29,respectively,P＜0.05).The protein expression profile of β1 integrin in these patients were similar to that of mRNA.Conclusions The expression of β1 integrin is decreased in the affected colon from the patients with HD,which may play a role in the pathogenesis of HD. Key words: Congenital aganglionosis;  Ganglion cell;  Integrin