Analysis on differential gene expression in shrimp Litopenaeus vannamei induced by IHHNV infection using high-throughput sequencing

【Objective】The differential gene expression in shrimp Litopenaeus vannamei induced by IHHNV infection was studied in order to provide references for further understanding the molecular interaction mechanisms of IHHNVshrimp interaction.【Method】The transcriptomes of the IHHNV-infected and control Litopenaeus vannamei were sequenced using the 454 high-throughput sequencing technology.After the removal of adaptors and sequence assembly by using the iAssembler software,unigenes(unique sequences) were obtained.Differentially expressed genes were determined by calculating the transcripts of each unigene(RPKM),and were validated by qRT-PCR.【Result】The sequencing produced a total of 208,690 short sequences,and the assembly generated 21,912 unigenes.Comparison between the sequencing data from IHHNV-infected and control Litopenaeus vannamei revealed 221 differentially expressed genes,including 81 upregulated genes and 140 down-regulated genes.qRT-PCR validation verified that the statistical gene expression results from 454 high-throughput sequencing data were realiable.【Conclusion】IHHNV infection had significant impact on immunerelated gene expression in Litopenaeus vannamei,while these differentially expressed genes could be detected using highthroughput sequencing.