Study on the injurious effect of a self designed micro-skin machine on the epithelia

Objective To observe the injury on micro-skin induced by a self designed micro-skin machine. Methods Micro-skin was produced either with the machine or by hand. Cells at the edge of micro-skin were observed by transmission electron microscope. succinic dehydrogenase activity in supernatant of cultivated cells was analyzed, and the cell proliferation of micro-skin was assessed by 3H-TdR. Twenty patients were enrolled in the study for the observation of the wound healing time between the two groups of microskin after being grafted. Results Transmission electron microscope examination revealed that the cellular injury at the edge of the micro-skin in machine-made group was mild compared with that in man-made group. 3H-TdR rate was elevated but the activity of succinic dehydrogenase in the supernatant of cultured cells decreased in supernatant of cultured cells of machine produced microskin. Wound healing time was shortened in machine made group.(P0.05). Conclusion The cellular injury at the edge of micro-skin in the machine made group was mild when compared with that in the manally group with cell proliferation accelerated and wound healing time shortened. [