Lactones from Ficus auriculata and their effects on the proliferation function of primary mouse osteoblasts in vitro

Abstract Bioassay-guided fractionation of the petroleum ether, chloroform and EtOAc extracts of the stems of Ficus auriculata led to the isolation of five new 12-membered lactones (3 R ,4 R )-4-hydroxy-de- O -methyllasiodiplodin ( 1 ), 6-oxolasiodiplodin ( 2 ) and ficusines A−C ( 3 – 5 ), together with three known related analogues ( 6 – 8 ). The structures of the new compounds were elucidated by comprehensive spectroscopic data. The absolute configurations of 3 and 8 were established by single crystal X-ray diffraction analysis. Compounds 3 – 5 represent the first 12-membered lactones with a quinone ring unit. Compounds 6 and 7 exhibited significant proliferation function of primary osteoblasts (OBs) in vitro. Especially, the promotion rate of 6 reached 151.55 ± 1.34% ( P