The effect of perilla oil on anti-aging for aging model rats

Objective: Study on the anti-aging effect of perilla oil on D-galactose induced aging model rats by the observation of physical ability, the ability of learning memory, antioxidant and lipid peroxidationindex, and research the anti-aging mechanism preliminarily. Methods: A total of 40 male SD rats of clean grade, 1 month old, were randomly divided into 5 groups. Negative control group, aging-model group, small dose perilla oil group (0.815 mL/kg), middle-dose perilla oil group (2.445 mL/kg), large-dose perilla oil group (7.337 mL/kg). Except for the negative control group were injected with saline, the remaining rats in each group in the subcutaneous injection of D galactose (125 mg/kg). After 8 weeks’ feeding, weight loading swimming test and Morris water maze experiment was carried out, then determine the content of superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide synthase (NOS) and nitric oxide (NO). Results: ompare with aging model group, the time of swimming in small-dose perilla oil treatment group, middle-dose perilla oil treatment group and large-dose perilla oil treatment group were longer (P0.05); the escape latency of small-dose perilla oil treatment group, middle-dose perilla oil treatment group and large-dose perilla oil treatment group were less than model group (P0.05); the effective time ratio in small-dose perilla oil treatment group, middle-dose perilla oil treatment group and large-dose perilla oil treatment group was higher than model group (P0.05). The time of going through the platform in small- dose perilla oil treatment group had no difference. In middle-dose perilla oil treatment group and large- dose perilla oil treatment group, the effective time ratio and the time of going through the platform were remarkably higher than model group (P0.05); compared with model group. In all groups the SOD activity was remarkably higher than model group. Conclusion: Successfully copy the D-galactose subacute aging model; Perilla oil can improve learning memory ability of D-galactose subacute aging model, increasing SOD activity and decreasing MDA content, indicating that Perilla oil have anti-aging effect. Perilla oil anti- aging efficiency was operated by reducing the blood free radicals.