31P- and 1H-NMR spectra of Manduca sexta larvae parasitized by Steinrnema carpocapsae

In vivo 31 P-nuclear magnetic resonance (NMR) spectroscopy was used to study changes in phosphorus metabolites of the fifth instar Manduca sexta larva parasitized by Steinernema carpocapsae. The 31 P-NMR spectrum of the intact larva was shown to be composed of five signals attributable to such phosphometabolites as the α, β and γ phosphates of adenosine triphosphate at -10.3, -19.05, and -5.2 ppm, respectively; phosphate of phospho-arginine at -3.26 ppm; and the largest signal at 3.45 ppm including phosphates of inorganic phosphate (Pi) and phosphomonoesters: fructose-6-phosphate, trehalose-6-phosphate, α-glycerophosphate (α-GP) and phosphorylcholine