MULTIVARIATE CALIBRATION STRATEGY FOR REVERSED-PHASE CHROMATOGRAPHIC SYSTEMS BASED ON THE CHARACTERIZATION OF STATIONARY MOBILE PHASE COMBINATIONS WITH MARKERS

Abstract Calibration of chromatographic systems serves several purposes. One of the goals is the correction of retention values in order to make them less dependent on the stationary and mobile phases. Another goal is the transfer of retention values measured on one system to another system. A strategy serving this purpose is reviewed. An example is given which makes use of the latter strategy to correct for batch-to-batch variations of stationary phases. This leaves the way open to update the optimum mobile phase composition. The strategy makes use of special selected compounds: the markers.