Antibiotic Resistance Patterns of E. coli and Staphylococcus aureus Isolated from Hospital wastewater samples of Mysore, Karnataka, South India

This study has been carried out to understand the antibiotic resistance patterns of commonly inhabits bacteria like Staphylococcus aureus and gram-negative bacteria E. coli were analyzed from hospital wastewater effluents. Twelve commonly used Pharmaceutical Compounds (PCs) were selected for this study. The PC’s selected for this study were Methicillin (MET), Cephalothin (CEP), Cefazolin (CZ), Cotrimoxazole (COT), Clindamycin (CD), Fusidic acid (FC), Oxicillin (OX), Linezolid (LZ), Faropenem (FAR), Doxycycline (DO), Vancomycin (VA)  and Rifamycin (RIF). The resistance, susceptible and intermediate patterns by the gram-positive and gram-negative bacteria towards the selected PC’s were observed. Among the tested antibiotics the gram-positive bacteria, Staphylococcus aureus showed 100% resistance towards the antibiotics of Methicillin (MET), Cephalothin (CEP), Cefazolin (CZ), Cotrimoxazole (COT), Clindamycin (CD), Fusidic acid (FC) and Rifamycin (RIF). The study result shows 30% resistance towards Faropenem (FAR) antibiotic. The gram-negative bacteria E. coli even showed 100% resistance towards Methicillin (MET), Cephalothin (CEP), Cefazolin (CZ), Cotrimoxazole (COT), Clindamycin (CD), Fusidic acid (FC) and Rifamycin (RIF). In general the selected PC’s it showed 70% resistance. The source water to the hospital effluent is contaminated with multiple drug resistant coliform bacteria and detection of Staphylococcus spp.