Tyrosinase inhibitory activity of Garcinia xanthochymus fruit pericarp extract

The tyrosinase is the main enzyme involved in melanin biosynthesis. The amount of melanin produced by melanocyte cells will affect a person's skin color. Therefore, the body's skin needs a compound that can inhibit the work of the enzyme tyrosinase. The purpose of this study was to determine the potential of G. xanthochymus fruit extracts as a whitening agent. The dried sample was continuously macerated using n-hexane and methanol. Furthermore, each extract's tyrosinase enzyme inhibitory activity was measured using a microplate reader 96 well spectrophotometers at 490 nm and a substrate of 3,4-dihydroxy-L-phenylalanine, L-D OPA. The results showed that the results of the Inhibitory Activity test at a concentration of 100 ppm from each extract were 35.41±11.10% (hexane extract), and 28.83 ± 6.77% (methanol extract). Percentage inhibition of the positive control (kojic acid) was 65.07 ± 0.03 % at 100 ppm. The conclusion of this research is that G. xanthochymus pericarp hexane and methanol extract have the potential as skin lightening with hexane extract results better than methanol extract, with inhibitory activity of tyrosinase enzyme half of kojic acid.