Four new sesquiterpenoids with anti-inflammatory activity from the stems of Jasminum officinale

Abstract Four new sesquiterpenoids，including three nor-cinalbicane type sesquiterpenoids, named Jasminol A, G, H ( 1–3 ) and one eremophilene-type sesquiterpenoid, named Jasminol B ( 4 ) together with nine known compounds ( 5–13 ) were isolated from the stems of Jasminum officinale L. The structures of new compound were elucidated on the base of IR, HR-ESI-MS, 1D NMR, 2D NMR and DEPT analysis, and the absolute configurations were determined by single-crystal X-ray diffraction analysis. In addition, the anti-inflammatory activity of isolated compounds was evaluated using lipopolysaccharide (LPS)-induced murine macrophage RAW264.7, and compounds 1 – 4 exhibited a moderate inhibition of LPS-induced nitric oxide (NO) production in RAW264.7 cells with IC 50 values of 20.56 ± 1.31, 30.12 ± 0.89, 30.35 ± 2.72 and 31.60 ± 1.69 μM, respectively, and CC 50 values >200 u M.