Growth hormone is a weaker candidate than prolactin for the hormone responsible for the development of a larval-type feature in cultured bullfrog skin
2003
SUMMARY Prolactin (PRL) has, for some years, been considered to be the `juvenile
hormone9 in amphibians. Recently, growth hormone (GH) has been proposed as
another candidate, because in the larval stages the expression of the mRNA GH
is high but it is downregulated in the climax stages of metamorphosis or
following treatment with thyroid hormone. In the present study, we
investigated whether GH promotes the development of one particular larval-type
feature of bullfrog tadpole skin in vitro . The amiloride-,
acetylcholine- and ATP-stimulated short-circuit current (SCC) is a
physiological marker of larval-type bullfrog skin. These types of
ligand-stimulated SCC (1) developed when EDTA-treated tadpole skin was
cultured with corticoids supplemented with PRL or GH and (2) were not
significantly different between skin cultured with PRL and intact tadpole
skin. However, the amiloride-induced SCC response in skin cultured with GH
differed in its kinetics from that of the intact (control) tadpole. On this
basis, PRL seems a better candidate than GH for the juvenile hormone, at least
with regard to the development of amiloride-stimulated non-selective cation
channels.
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