Механизмы апоптоза в моноцитарно-фагоцитарной системе при развитии адъювантного артрита после введения клеток фетальной печени

Comparative evaluation of apoptotic processes in cells of monocytic-phagocytic system in the development of adjuvant arthritis before and after the introduction of cryopreserved and native fetal liver cells is done. Western blot analysis showed that under the conditions of adjuvant arthritis development the cells of monocytic-phagocytic system are resistant to apoptosis. Proapoptotic effect of fetal liver cells is set followed by their administration to the recipients with adjuvant arthritis that was more pronounced in the cryopreserved fetal liver cells. Development of an apoptosis inducible potential of fetal liver cells directly depended both on the type of drugs and term of their treatment. The nature of CD95-cascade protein expression in cells of monocytic-phagocytic system after fetal liver cells treatment emphasized cryopreservation effect on functional status of a biological object. In this case cryopreservation might appear as protein activation factor of a proapoptotic cascade. It is noted that the mechanism of action of fetal liver cells in modulation of apoptotic cascades in the cells of the monocyte-phagocytic animal pathology is significantly different from the action of glucocorticoids used in rheumatoid arthritis treatment, which is an experimental model of adjuvant arthritis. These results are experimental basis for the possibility to use fetal liver cells for rheumatoid arthritis treatment.