Data Independent Acquisition in Practice: Foundations and Resources for Implementing DIA.

: Proteomics researchers are realizing the promise of Data-Independent Acquisition (DIA). In its 2017/2018 study, the ABRF Proteomics Research Group (PRG) have conducted a worldwide study to offer a go-to resource for sample processing, data acquisition and data analysis for new users interested in DIA studies. This workshop will facilitate an in-depth discussion of the PRG study results, and provide practical demonstrations utilizing those created during the PRG study. Workshop Goals: Educate and provide online educational resources for DIA that aid in the areas of sample preparation, data acquisition, data analysis and interpretation; enable academic and core facilities to use DIA technology by providing expert insights and possible improvements to existing workflows; share experience with attendees on some common lessons learnt during the PRG study; and lay the foundation for attendees to implement DIA at their own institution.