Control of contamination during micropropagation process of Rootstocks Mariana (Prunus mariana)

Mariana (Prunus mariana) is one of the most important Root stocks of plum that is propagated by tissue culture. The problem of using explants of these Root stocks is contamination with fungus and bacteria and preparation of sterile explants is difficult. In this research, effects of different sterilizer compounds such as Sodium hypochlorite and 70% Ethanol in different concentrations and periods on contamination control of Mariana Root stocks were investigated. Bud establishing were done in MS medium. After four weeks percentages of budding, emerging and contamination were measured for different treatments. Results showed that using of Antibiotics in sterilizing process of explants with 10% Sodium hypochlorite, enhanced contamination control. Also the use of Cefotaxime Antibiotic (400 mg/l) in comparison with combination of Carbenicillin and Streptomycin antibiotics (each 200mg/l) is more effective on contamination control.