Isolation and biological properties of polysaccharide CPS-1 from cultured Cordyceps militaris.

Abstract A polysaccharide from the water extract of cultured Cordyceps militaris was isolated through ethanol precipitation, deproteination and gel-filtration chromatography. Their molecular weight was determined using gel-filtration chromatography. The structure of polysaccharide CPS-1 was elucidated by sugar analysis, Smith degradation, IR and 13 C–NMR spectroscopy. CPS-1 was shown to possess a significant antiinflammatory activity and suppressed the humoral immunity in mice but had no significant effects on the cellular immunity and the non-specific immunity.