Novel culture medium for producing pulullan and method for fermenting and producing pulullan
2012
The invention relates to a novel culture medium and production technique for fermenting and producing pulullan. The formula of the culture medium is as follows: sucrose is used as a carbon source, the concentration of the sucrose is 60-120g/L, urea is used as a nitrogen source, and the concentration of the urea is 2-4g/L; K2HPO4, MgSO4.7H2O, and FeSO4.7H2O are used as inorganic salts, the concentration of the NaCl is 2-4g/L, the concentration of the K2HPO4 is 3-10g/L, the concentration of the MgSO4.7H2O is 0.3-0.5g/L, the concentration of the FeSO4.7H2O is 25-100mg/L, and the pH ranges from 6 to 7. The culture medium has clear ingredients, the ingredients of the fermented product are simple, and the following extraction production cost can be lowered; and the culture medium is wide for ingredient source, easy to buy and lower in cost.
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