SCREENING FOR POTENTIAL ANTIMCROBIAL COMPOUNDS FROM GANODERMABONINENSE AGAINST SELECTED FOOD BORNE AND SKIN DISEASE PATHOGENS

Objective: The present study aims to investigate the potential antimicrobial compounds from the fungi against selected foodborne and skin disease pathogens. Methods: In this study, four different types of solvents (hexane, chloroform, dichloromethane and methanol) were used to screen the potential antimicrobial compounds from the G. boninense fruiting bodies. The antimicrobial activity of the G. boninense crude extract was studied against some common food borne and skin diseases bacterial pathogens such as Escherichia coli, Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa, Streptococcus pyogenes, Streptococcus pneumoniae, Staphylococcus aureus and Klebsiella spp. using disc diffusion assay. Results: Crude extraction showed methanolic extraction produced the highest yield (2.61%) followed by chloroform (1.36%) and dichloromethane (0.50%). The lowest yield obtained was from hexane extraction (0.17%). Antimicrobial study revealed that methanol and chloroform extracts showed broad spectrum activity to all tested pathogens with inhibition ranging from7.8-11.3 ± 0.0-1.0 mm and 6.8-8.3 ± 0.0-1.0 mm respectively. A clear inhibition zone where Aspergillusniger failed to developed on TLC platesdipped in chloroform: ethyl acetate (95:5, v/v) at Rf 0.33, 0.40 and 0.69 was also observed using chloroform crude extract. GC-MS results confirmed G. boninense contains bioactive compoundssuch as dodecanoic acid, cyclododecane, octadecanoic acid, 9-octadecenoic acid, hexadecanoic acid, methyl tetradecanoate, 9, 12-octadecadienoic acid, dodecyl acrylate and hexadecanoic acid. Conclusion: G. boninensecontains many bioactive compounds which are potential to be further explored as an antimicrobial agent against food borne and skin diseases pathogens.