Study on TCRVβ gene expression of rats with dampness syndrome

Objective:To investigate variety pattern of expression level of TCRVβsubfamilies in mononuclear cell in spleen tissue of rats with dampness pathogenic factors and normal rats by using FQ-PCR technique. Methods:32 SD rats were divided into four groups: normal group, external dampness group, internal dampness group, external and internal dampness group. Observing period was 20 days. 3 Rats were randomly selected from each group in order to exam the TCRVβ subfamilies expression level. Results:The expression of TCRVβ1, TCRVβ7, TCRVβ9 and TCRVβ13 in external dampness group were higher than those in normal group (P0.05,P0.01,P0.001). The expression of TCRVβ8, TCRVβ14 and TCRVβ18 in internal dampness group were lower than those in normal group (P0.05,P0.01), also the expression of TCRVβ8 and TCRVβ18 in internal and external dampness group were lower than those in normal group (P0.05,P0.01). Conclusion:The expression of TCRVβsubfamilies in dampness groups was disordered, several TCRVβ expression in external group were higher while several TCRVβ expression in internal dampness group and both internal and external group were lower than those in normal group, which mean the hoodwinks nature of dampness evil that might further cause immune disorder in model groups.