Sensitivity to taxoid derivatives of a newly established human endometrioid ovarian adenocarcinoma radioresistant cell line.

An endometrioid ovarian adenocarcinoma cell line CAVEOC-2 was characterized. Maintained in monolayered culture, CAVEOC-2 cells exhibited a 33-hr doubling time. When xenografted into nude mice, these cells produced fast growing tumors. Colony-forming efficiency in agar was 50%. DNA index was 1.5 and cytogenetic analysis showed a triploid karyotype. CAVEOC-2 cells did not express mdr-1 gene and were chemosensitive to doxotubicin (IC50=1.82±0.76 μmol/l), paclitaxel (IC50 = 3.33±0.26 nmol/l) and docetaxel (IC50 = 0.68±0.28 nmol/l), while they showed an intermediate sensitivity to cisplatin (IC50 = 9.40±1.02 μmol/l). CAVEOC-2 cells seemed highly radioresistant (SF 2 =0.81, α=0.02 Gy -1 , β=0.025 Gy -2 , and MID =4.31 Gy). Activities of glutathione S transferase and γ-glutamyl transpeptidase were respectively 23.5- and 3.4-fold higher than those of sensitive A2780 cell line. These characteristics make the CAVEOC-2 cells a suitable model for the study of human endometrioid ovarian adenocarcinoma.