Cloning and sequence analysis of P_(38) gene of Grapevine fanleaf virus Hangzhou isolated.

To confirm the characteristics and function of P38 in the viral particles movement, the cDNA fragment of P38 gene of Hangzhou isolate of Grapevine fanleaf virus (GFLVH) was amplified by RTPCR with specific primers to the RNA2 sequence of GFLVF13 and was then inserted into pGEMTeasy vector. The result of sequence analysis indicated that this gene contained 1044 nucleotides encoding 347 amino acids and were 90% and 96% identical to GFLVF13 at nucleotide and amino acid level, respectively. Multiple sequence alignment of amino acid sequence showed that homology between P38 gene of GFLVH and that of Arabis mosaic virus (ArMV) and that of Tomato ringspot virus (TomRSV) was 85% and 38.6%, respectively, while its homology with Tabacco black ring virus (TBLV), Grapevine chrome mosaic virus (GCMV) and Raspberry ringspot virus (RRSV) was below 30%.