Isolation and variability of polymorphic microsatellite loci in Aedes aegypti, the vector of dengue viruses

We isolated five microsatellite sequences from an enriched-(CAA)n library of 5000 recombinant clones in Aedes aegypti. Two polymorphic microsatellites from our library and four from other sequence databases were tested: we investigated their polymorphism and Mendelian inheritance in mosquito populations. Our results indicate that trinucleotide repeat markers could be used to differentiate Ae. aegypti populations, making them valuable tools for the study of population genetic structure.