Effect of SLC7A5 on cell proliferation in gastric cancer and its pathologic significance

Objective:To investigate the transcription and expression of SLC7A5 in GC tissue and cell lines,and analyze the influence of SLC7A5 on GC cell proliferation and its clinicopathologic significance.Methods:Levels of SLC7A5 expression in GC tissues and match paired non-cancerous tissues from 52 patients were detected by immnohistochemistry analysis.Differences in transcription and protein expression of SLC7A5 between GC cell lines(SGC-7901,MKN-45,MGC-803 and CRL-5974) and human gastric epithelial immortalized GES-1 cells were surveyed.Correlations between the expression of SLC7A5 and the clinicopathologic features of GC were analyzed.GC SGC-7901 cells,in which expression of SLC7A5 was down-regulated by pGU6/ GFP/Neo/sh-RNA,were studied for cell proliferation ability and cell cycle by applying CCK assay and flow cytometry.Results:The expression of SLC7A5 in GC tissues was up-regulated and associated significantly with tumor size,local invasion,lymph node metastasis and advanced TNM stage.Down-regulation of SLC7A5 expression in SGC-7901 cells inhibited the ability of cell proliferation,along with significant G0/G1 cell cycle arrest.Conclusions:SLC7A5 confers GC cells with greater proliferation and is associated with the clinicopathologic features of GC,including tumor size,local invasion,lymph node metastasis and TNM stage.SLC7A5 could be a biomarker for predicting GC biological behavior,and perhaps become a new target for GC diagnosis and treatment.