Female Hormone 17β-Estradiol Downregulated MMP-2 Expression and Upregulated A1PI Expression in Human Corneal Stromal Cells

Collagens are essential for cornea functions. In non-ocular tissues, it has been demonstrated that sex hormones modulate the collagen remodeling. In this study, we investigated whether the primary female hormone 17β-estradiol plays a role in the expressions of matrix metalloproteinases and proteinase inhibitors in cultured human corneal stromal cells. We found that 17β-estradiol treatment significantly reduced the matrix metalloproteinase-2 mRNA in human corneal stromal cells as well as the matrix metalloproteinase-2 proteins, while the matrix metalloproteinase-9 mRNA level was not significantly altered. 17β-estradiol also upregulated the expression of proteinase inhibitor, alpha1-proteinase inhibitor. The expression of transcription factor specificity protein 1 was reduced by 17β-estradiol. Furthermore, 17β-estradiol did not change the viability and apoptosis of the corneal stromal cells. The downregulation of matrix metalloproteinase-2 and upregulation of alpha1-proteinase inhibitor by 17β-estradiol possibly serve as protective factor for the normal tomography in antagonizing the extracellular matrix degeneration in many cornea diseases.