Development of a polarization imaging method to detect paraffin-embedded pathology tissues before applying other techniques.

The present paper describes the development of a technique, applied to paraffin-embedded tissues, which uses three different wavelengths of monochromatic light (λ1=445nm, λ2=540nm, and λ3=660nm) for the measures of the Degree of Polarization (DoP), Degree of Linear Polarization (DoLP), Degree of Circular Polarization (DoCP) and birefringence, all obtained from measurements of Stokes parameters by using polarized light. The goal of this study was to detect changes in developing embryonic mouse eye when pregnant mice fed diets without folic acid (FA) for variable periods compared with a healthy control group. We present a biomedical diagnostic technique based on polarized light detection applied to paraffin-embedded tissues to visualize the structural damage to aid us in the diagnosis before applying other techniques. Through this method, we can visualize and identify which parts of the tissue were altered with respect to the control group. This article is protected by copyright. All rights reserved.