Quantitative RP–LC Analysis of Mangiferin and Homomangiferin in Mangifera indica L. Leaves and in Mangifera persiciforma C.Y. Wu et T.L. Ming Leaves

Mangiferin is a pharmacologically active flavonoid, a natural xanthone C-glycoside. Homomangiferin is mangiferin monomethyl ether. Mangiferin and homomangiferin have important medicinal properties and are widely used to relieve many symptoms, for example coughing and asthma. A new gradient reversed-phase high-performance liquid chromatographic method has been developed for analysis of these compounds. The amounts of mangiferin and homomangiferin in 20 samples of Mangifera indica L. leaves and in five samples of M. persiciforma C.Y. Wu et T.L. Ming leaves were quantified by use of the method. The method was validated for system suitability, precision, accuracy, and linearity. The average recovery of the method at three levels was 98.7–101.5% and linearity was good (r > 0.9998) for both mangiferin and homomangiferin over a relatively wide range of concentration. The amounts of the two flavonoid compounds in M. indica L. leaves of different species or collected in different months, and in M. persiciforma C.Y. Wu et T.L. Ming leaves from different collection sites were evaluated in this study and the differences are discussed.