Development and cytogenetic characterisation of a double goat grass-barley chromosome substitution in tritordeum

×Tritordeum (Ascherson et Graebner, an amphiploid between Triticum turgidum conv. durum and Hordeum chilense), and chromosome substitution lines of tritordeum where chromosomes 2 Hch or 3 HchH. chilense were replaced with chromosome 2 D of T. aestivum or 3 Hv chromosome of H. vulgare, respectively, were used to assess the effect of specific chromosomes on the rachis. ×Tritordeum has brittle rachis while the 2 D(2 Hch) and 3 Hv (3 Hch) substitution lines have non-brittle rachis. Both lines also have compact spikes, a character highly desirable for the improvement of tritordeum threshability. Different combinations of 2 D and 3 Hv translocations were developed in tritordeum. In this article we present information on the identification and characterisation of all these introgression lines by the fluorescent in situ hybridisation.