Anti-Allergic Catechins of TEA(Camellia Sienesis)

Several in vitro and in vivo studies have demonstrated that components of tea (Camellia Sinensis L) exhibit biological and pharmacological properties. Anti­allergic effect is one of these functional properties in which catechins apparently play a significant role. We report the anti-allergic effect of epigallocatechin-3-O-(3-O­-methyl) gallate (EGCG3”Me) and epigallocatechin-3-O-(4-O-methyl) gallate (EGCG4”Me) isolated from Japanese (Assam hybrid) or Taiwanese tea leaves. Oral administration of these methylated catechins significantly and dose-dependently (5–50 mg/kg) inhibited type I allergic (anaphylactic) reactions in mice sensitized with ovalbumin and Freund’s incomplete adjuvant. And these catechins strongly inhibited mast cell activation through the prevention of tyrosine phosphorylation (Lyn, Syk and Btk) of cellular protein and histamine/leukotrienes release , interleukin-2 secretion after Fcepsolon RI cross-linking. Furthermore, from the investigation that the effects of varieties, tea seasons of crops and manufacturing methods, to benefit the anti-allergic effect of EGCG3”Me, green or semi-fermented teas, made from Benihomare, Benifuuki and Benifuji at second crop onwards, should be consumed.