Major Microorganisms Involved in Nitrogen Cycle in Plateau Cold Region and Its Relationship with Environmental Factors

In order to find the major microorganisms involved in nitrogen cycle and analysis the relationship with environment factors in plateau cold region, the representative soil types in the Huangshui river basin (above Xining city) were sampled. High-throughput sequencing was used to study the diversity of soil bacteria, and the composition and abundance of bacteria in the samples were analyzed. With the help of significance test regression analysis, the correlation between species abundance and environmental factors was analyzed, and the spatial distribution and causes of soil nitrogen were further analyzed. The results showed that major microorganisms main genera involved in nitrogen cycle are Nitrospira Bacillus, Nocardioides and Bradyrhizobium. And there was a negative correlation between soil nitrogen content and elevation, a significant negative correlation between Bradyrhizobium and soil TN, and a negative correlation between Nitrospira and Nocardioides species abundance. This study makes a preliminary exploration on major microorganisms involved in nitrogen cycle in plateau cold region and its relationship with environmental factors.