dehydrogenase 2 is associated with diminished enzyme activity

-monophosphatedehydrogenase 2 is required for purine synthesis inactivated lymphocytes. Variants in the IMPDH2 gene mayaccount for the large inter-individual variability in baselineenzyme activity, immunosuppressive efficacy and sideeffects in transplant recipients receiving mycophenolicacid. Therefore, the objective of this study was to identifyand functionally characterize IMPDH2 variants.Methods DNA samples from 152 solid organ transplantpatients were screened at exons and exon/intron junctionsof the IMPDH2 genes by PCR amplification followed bybidirectional direct DNA sequencing. Genetic variant wasconstructed by site-directed mutagenesis and transformedto an inosine 5