Nasal polyposis: A semiquantitative morphometric histopathological study

Background / Aim. Nasal polyps are inflammatory hypertrophic proliferations of the sinonasal mucosa composed of both epithelial and stromal elements. The aim of this study was to determine histopathological hallmarks of nasal polyposis via semiquantitative morphometric study. Methods. Study was designed as prospective study with semiquantitative morphometric analysis and it comprised 77 patients with chronic rhinosinusitis and nasal polyposis that underwent functional endoscopic sinonasal surgery performed by the same surgeon. Morphometric analysis included gradation of tissue edema within polyps, thickening of epithelial basal membrane, degree of inflammation, presence/absence of metaplasia within epithelium, degree of fibrosis within polyps, and percentage of inflammatory cells within inflammatory infiltrate (lymphocytes, macrophages, plasma cells, neutrophils and eosinophils). Results. As expected, samples from study group showed significantly higher degree of inflammation than samples from control group (χ2 =35.89 with p value less than 0.01). Degree of fibrosis in nasal polyposis is in positive correlation with duration of symptoms (r = 0.25, p < 0,05) and with percentage of macrophages in inflammatory infiltrate (r = 0.26, p < 0.05).. Patients with NP had significantly lower number of lymphocytes (r=-7.66, p < 0.01), but significantly higher number of eosinophils (r=3.84, p < 0.01), macrophages (r=3.34, p < 0.01) and plasma cells (r=3.14, p < 0.01) than controls, with p value less than 0.01. Conclusion. Tissue samples from patients with nasal polyposis show significant changes that reflect in various degrees of inflammation, fibrosis and basement membrane thickening which may contribute to more difficult surgical management and perioperative complications such as bleeding.