[Effect of NF-κB on the expression of interleukin-6 induced by lipopolysaccharides of Porphyromonas endodontalis in MC3T3-E1 cells].

PURPOSE: To investigate the effect of NF-κB signaling on the expression of interleukin-6(IL-6) induced by lipopolysaccharides(LPS) extracted from Porphyromonas endodontalis(P.e)in MC3T3-El cells.METHODS: MC3T3-E1 cells were pretreated with BAY-117082 for 1 h,and then were treated with 10 mg/L P.e-LPS for different times.The translocation of NF-κB was observed by immunofluorescence.The expression of IL-6 was detected by reverse transcription polymerse chain reaction(RT-PCR) and enzyme-linked immuno sorbent assay(ELISA).Statistical analysis was performed using multi-way ANOVA and Dunnett’s t test with SPSS 13.0 software package.RESULTS: The staining of NF-κB was mostly in cytoplasm in untreated cells.Rapid translocation of NF-κB into nucleus was observed in the cells stimulated for 30 min and mostly relocalization of NF-κB from nucleus to cytoplasm was observed after 60 min.Pretreatment with 10 μmol/L BAY-117082 for 1h significantly inhibited P.e-LPS-induced translocation of NF-κB.The mRNA and proteins of IL-6 decreased significantly after pretreatment with 10 μmol/L BAY-117082,and the expression of IL-6 proteins was reduced from(774.983±6.585) ng/L to(377.384±14.620) ng/L(P0.01).The group of treatment with BAY-117082 alone had no significant difference from the blank control group.CONCLUSIONS: P.e-LPS can induce translocation of NF-κB in mouse osteoblast MC3T3-El,and P.e-LPS may induce the expression of IL-6 in mouse osteoblast through the signaling of NF-κB.Supported by Science and Technology Program of Liaoning Province(2011225020).