Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies.

Antibodies bearing engineered cysteine residues (termed THIOMAB antibodies) enable the site-selective attachment of a drug, label or other payload for specific delivery to certain tissues (e.g., tumors). This Chapter describes detailed methods we have developed and optimized for the conjugation, purification and analysis of THIOMAB antibody drug conjugates (TDCs).